Variable Incidence Angle Fluorescence Interference Contrast Microscopy for Z-Imaging Single Objects

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Variable incidence angle fluorescence interference contrast microscopy for z-imaging single objects.

Surface-generated structured illumination microscopies interrogate the position of fluorescently labeled objects near surfaces with nanometer resolution along the z axis. However, these techniques are either experimentally cumbersome or applicable to a limited set of experimental systems. We present a new type of surface-generated structured illumination fluorescence microscopy, variable incide...

متن کامل

Effective elimination of laser interference fringing in fluorescence microscopy by spinning azimuthal incidence angle.

Laser illumination used in both conventional widefield epi-fluorescence as well as in total internal reflection fluorescence (TIRF) microscopy is subject to nonuniformities in intensity that obscure true image details. These intensity variations are interference fringes arising from coherent light scattering and diffraction at every surface in the laser light's optical path, including the lense...

متن کامل

Virtual Supercritical Angle Fluorescence Microscopy for Real Time Membrane Imaging

We present an alternative full field imaging technique based on Supercritical Angle Fluorescence (SAF). When fluorescent emitters are placed in the vicinity of the glass slide, their near-field components become propagative at supercritical angles. This supercritical emission decays sharply the fluorophore/surface distance d with a characteristic decay length of about 100 nm. Selecting the supe...

متن کامل

Full-field supercritical angle fluorescence microscopy for live cell imaging.

We introduce a full-field fluorescence imaging technique with axial confinement of about 100 nm at the sample/substrate interface. Contrary to standard surface imaging techniques, this confinement is obtained through emission filtering. This technique is based on supercritical emission selectivity. It can be implemented on any epifluorescence microscope with a commercial high numerical aperture...

متن کامل

Fluorescence interference-contrast microscopy of cell adhesion on oxidized silicon

Standing modes of light in front of the reflecting surface of silicon modulate the excitation and emission of fluorescent dyes. This effect was used to determine the distance of a biomembrane from an oxidized silicon chip. The membrane of a red blood cell (ghost) was stained with a cyanine dye and attached with poly-lysine to a surface structured with microscopic steps of silicon dioxide on sil...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Biophysical Journal

سال: 2005

ISSN: 0006-3495

DOI: 10.1529/biophysj.105.066738